Temporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolations
Identifieur interne : 000398 ( France/Analysis ); précédent : 000397; suivant : 000399Temporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolations
Auteurs : Andreas Meyerhans [France] ; Rémi Cheynier [France] ; Jan Albert [Suède] ; Martina Seth [France] ; Shirley Kwok [États-Unis] ; John Sninsky [États-Unis] ; Linda Morfeldt-M Nson [Suède] ; Birgitta Asjö [Suède] ; Simon Wain-Hobson [France]Source :
- Cell [ 0092-8674 ] ; 1989.
English descriptors
- Teeft :
- Abrupt changes, Aids virus, Amino acid sequence, Amino acid substitutions, Amplification, Background value, Base substitutions, Biological properties, Buffer gradient, Cell count, Cell lines, Clone, Codon, Copy number, Data sets, Defective, Defective genomes, Dominant form, Dominant forms, Dynamic role, Envelope gene, Equimolar mixture, Fetal calf serum, First coding exon, Gene, Gene expression, Genetic variation, Genome, High frequencies, Hindlll, Human immunodeficiency virus, Hybridization, Immune responses, Immunodeficiency, Immunodeficiency syndrome, Internal hindlll, Kpnl, Kpnl sites, Lymphocyte, Major form, Major forms, Major sequences, Mepvdprlepwkhpgsqpkt acnncyckkccfhcqvcftkkglgisygrk krrqrrrapq dsethqvslskq, Minor form, Minor forms, Molecular terms, Nucleic, Nucleic acid seqs, Nucleic acid sequences, Pbmc, Peripheral blood, Plasmid, Point agarose, Polymerase, Polymerase chain reaction, Protein sequence, Protein sequences, Provirus, Quasispecies, Recherche medicale, Recombinant, Remi cheynier, Replication, Room temperature, Sequential, Single adenosine residue, Small sample, Southern blots, Subsequent figures, Substitution, Such complexity, Tissue culture conditions, Unpublished data, Viral, Viral populations, Virol, Virus, Vivo, Year period.
Abstract
Abstract: A genetic study has been made of the HIV tat gene from sequential HIV-1 isolates and the corresponding infected peripheral blood mononuclear cells. DNA was amplified by polymerase chain reaction (PCR) and cloned into a eukaryotic expression vector. Twenty clones were sequenced from each sample. Comparing the sequential HIV isolates, abrupt differences were seen between the major forms of each isolate. These progressive changes were not reflected at all among the in vitro samples. The fluctuation in the quasispecies in vivo may suggest a much more dynamic role for Iatently infected mononuclear cells. High frequencies of functionally defective tat genes were identified. Given such complexity and the evident differences between quasispecies in vivo and in vitro, the task of defining HIV infection in molecular terms will be difficult.
Url:
DOI: 10.1016/0092-8674(89)90942-2
Affiliations:
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sortKey="Morfeldt M Nson, Linda" sort="Morfeldt M Nson, Linda" uniqKey="Morfeldt M Nson L" first="Linda" last="Morfeldt-M Nson">Linda Morfeldt-M Nson</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology Karolinska Institute Stockholm</wicri:regionArea></affiliation></author><author><name sortKey="Asjo, Birgitta" sort="Asjo, Birgitta" uniqKey="Asjo B" first="Birgitta" last="Asjö">Birgitta Asjö</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology Karolinska Institute Stockholm</wicri:regionArea></affiliation></author><author><name sortKey="Wain Hobson, Simon" sort="Wain Hobson, Simon" uniqKey="Wain Hobson S" first="Simon" last="Wain-Hobson">Simon Wain-Hobson</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie et Immunologie Moléculaires des Rétrovirus Institut Pasteur 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lines</term><term>Clone</term><term>Codon</term><term>Copy number</term><term>Data sets</term><term>Defective</term><term>Defective genomes</term><term>Dominant form</term><term>Dominant forms</term><term>Dynamic role</term><term>Envelope gene</term><term>Equimolar mixture</term><term>Fetal calf serum</term><term>First coding exon</term><term>Gene</term><term>Gene expression</term><term>Genetic variation</term><term>Genome</term><term>High frequencies</term><term>Hindlll</term><term>Human immunodeficiency virus</term><term>Hybridization</term><term>Immune responses</term><term>Immunodeficiency</term><term>Immunodeficiency syndrome</term><term>Internal hindlll</term><term>Kpnl</term><term>Kpnl sites</term><term>Lymphocyte</term><term>Major form</term><term>Major forms</term><term>Major sequences</term><term>Mepvdprlepwkhpgsqpkt acnncyckkccfhcqvcftkkglgisygrk krrqrrrapq dsethqvslskq</term><term>Minor form</term><term>Minor forms</term><term>Molecular terms</term><term>Nucleic</term><term>Nucleic acid seqs</term><term>Nucleic acid sequences</term><term>Pbmc</term><term>Peripheral blood</term><term>Plasmid</term><term>Point agarose</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Protein sequence</term><term>Protein sequences</term><term>Provirus</term><term>Quasispecies</term><term>Recherche medicale</term><term>Recombinant</term><term>Remi cheynier</term><term>Replication</term><term>Room temperature</term><term>Sequential</term><term>Single adenosine residue</term><term>Small sample</term><term>Southern blots</term><term>Subsequent figures</term><term>Substitution</term><term>Such complexity</term><term>Tissue culture conditions</term><term>Unpublished data</term><term>Viral</term><term>Viral populations</term><term>Virol</term><term>Virus</term><term>Vivo</term><term>Year period</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A genetic study has been made of the HIV tat gene from sequential HIV-1 isolates and the corresponding infected peripheral blood mononuclear cells. DNA was amplified by polymerase chain reaction (PCR) and cloned into a eukaryotic expression vector. Twenty clones were sequenced from each sample. Comparing the sequential HIV isolates, abrupt differences were seen between the major forms of each isolate. These progressive changes were not reflected at all among the in vitro samples. The fluctuation in the quasispecies in vivo may suggest a much more dynamic role for Iatently infected mononuclear cells. High frequencies of functionally defective tat genes were identified. Given such complexity and the evident differences between quasispecies in vivo and in vitro, the task of defining HIV infection in molecular terms will be difficult.</div></front></TEI><affiliations><list><country><li>France</li><li>Suède</li><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="France"><noRegion><name sortKey="Meyerhans, Andreas" sort="Meyerhans, Andreas" uniqKey="Meyerhans A" first="Andreas" last="Meyerhans">Andreas Meyerhans</name></noRegion><name sortKey="Cheynier, Remi" sort="Cheynier, Remi" uniqKey="Cheynier R" first="Rémi" last="Cheynier">Rémi Cheynier</name><name sortKey="Seth, Martina" sort="Seth, Martina" uniqKey="Seth M" first="Martina" last="Seth">Martina Seth</name><name sortKey="Wain Hobson, Simon" sort="Wain Hobson, Simon" uniqKey="Wain Hobson S" first="Simon" last="Wain-Hobson">Simon Wain-Hobson</name></country><country name="Suède"><noRegion><name sortKey="Albert, Jan" sort="Albert, Jan" uniqKey="Albert J" first="Jan" last="Albert">Jan Albert</name></noRegion><name sortKey="Albert, Jan" sort="Albert, Jan" uniqKey="Albert J" first="Jan" last="Albert">Jan Albert</name><name sortKey="Asjo, Birgitta" sort="Asjo, Birgitta" uniqKey="Asjo B" first="Birgitta" last="Asjö">Birgitta Asjö</name><name sortKey="Morfeldt M Nson, Linda" sort="Morfeldt M Nson, Linda" uniqKey="Morfeldt M Nson L" first="Linda" last="Morfeldt-M Nson">Linda Morfeldt-M Nson</name></country><country name="États-Unis"><region name="Californie"><name sortKey="Kwok, Shirley" sort="Kwok, Shirley" uniqKey="Kwok S" first="Shirley" last="Kwok">Shirley Kwok</name></region><name sortKey="Sninsky, John" sort="Sninsky, John" uniqKey="Sninsky J" first="John" last="Sninsky">John Sninsky</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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